Chiral metamaterial-based sensor is designed for sensing the change in substance ratio of chemical substances when combined with distilled water. These samples have been prepared with 10%, 50% and 90% volume fraction of methanol, ethanol, acetone, ammonia and isopropyl alcohol. The complex permittivity of the prepared samples has been measured by Agilent 85070E dielectric probe kit and compared with the simulations. In the proposed design, linear shifts with the frequency bands of more than 100 MHz are observed in different measurements. For different material sensing, pure samples have been used and their reflection coefficient measurement results have been presented. Unique side of this study is that the structure provides very clear and sensitive results and presents a new approach to the microfluidic sensor applications by using the sample holder.