Establishment of A Novel Plant Micropropagation System From Mature Hydroprimed Seeds Of Two Turkish Broad Bean Cultivars

TURAN F., KHAVAR K. M.

TURKISH JOURNAL OF AGRICULTURAL AND NATURAL SCIENCE, cilt.9, sa.2, 2022 (TRDizin)

Özet

  1. eguminous Broad bean (Viciafaba L.)is an excellent source of minerals, vitamins and proteins that makes it highly valuable food forhuman and feed for animal consumption. The plant has an important characteristicto fix atmospheric Nitrogenand play an important role to establish a natural balance of N in the atmosphere.It is a highly self-pollinatedplant and hasproblem of low variation withlimitedgeneticpool.Moreover, the previous studies identify problem of recalcitrance in broad bean.Therefore, thereis need to establish a repeatable micropropagation protocol that could ensure an increase in genetic variability to overwhelm problems in breeding.This system must also be able for efficient gene delivery and could be integrated with the conventional breeding programsthrough direct organogenesis. The study aimed to develop a tissue culture(shooting &rooting)protocol on two important Turkish broad bean cultivarsFiliz99 and Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mgl-1TDZusing embryonic axis as explant. Maximum number of 5.33 and 3 shoots per explant were noted on cv. Filiz99 and Eresen87 on MS medium containing 0.15 mg l-1TDZ. The developing shoots were rooted on MS medium containing 1 mg l-1IAA after threeweeks of culture. The rooted plants were transferred to pots containing peat under maintained under controlled greenhouse conditions for acclimatizationThe acclimatized plants bloomed and set seeds.Present results underscore importance of seed hydroprimingbefore taking of explants to achieve high micropropagation on faba beans to overcome recalcitrance.

    Broad bean (Viciafaba L.)is an excellent source of minerals, vitamins and proteins that makes it highly valuable food forhuman and feed for animal consumption. The plant has an important characteristicto fix atmospheric Nitrogenand play an important role to establish a natural balance of N in the atmosphere.It is a highly self-pollinatedplant and hasproblem of low variation withlimitedgeneticpool.Moreover, the previous studies identify problem of recalcitrance in broad bean.Therefore, thereis need to establish a repeatable micropropagation protocol that could ensure an increase in genetic variability to overwhelm problems in breeding.This system must also be able for efficient gene delivery and could be integrated with the conventional breeding programsthrough direct organogenesis. The study aimed to develop a tissue culture(shooting &rooting)protocol on two important Turkish broad bean cultivarsFiliz99 and Eresen87 using MS medium containing 0.05, 0.15, 0.25, 0.35, 0.45, 0.55 mgl-1TDZusing embryonic axis as explant. Maximum number of 5.33 and 3 shoots per explant were noted on cv. Filiz99 and Eresen87 on MS medium containing 0.15 mg l-1TDZ. The developing shoots were rooted on MS medium containing 1 mg l-1IAA after threeweeks of culture. The rooted plants were transferred to pots containing peat under maintained under controlled greenhouse conditions for acclimatizationThe acclimatized plants bloomed and set seeds.Present results underscore importance of seed hydroprimingbefore takin