Antimicrobial Activity of Ozone against Pathogenic Oral Microorganisms on Different Denture Base Resins

HAYRAN Y., Deniz S. T., AYDIN A.

Ozone: Science and Engineering, cilt.42, sa.1, ss.43-53, 2020 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/01919512.2019.1626215
  • Dergi Adı: Ozone: Science and Engineering
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, Compendex, Environment Index, INSPEC, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.43-53
  • Anahtar Kelimeler: acrylic resins, bacteria, candida albicans, cell survival, cell viability, denture bases, Ozone
  • Yozgat Bozok Üniversitesi Adresli: Evet

Özet

The present study aimed to evaluate the antimicrobial effect of gaseous ozone against specific oral pathogens on denture base resins. 1080 round samples were prepared (10mm-diameter, 2mm-thickness). Candida albicans, Streptococcus mutans, Streptococcus gordonii, and Aggregatibacter actinomycetemcomitans, polyamide-Deflex, heat-cured polymethyl-methacrylate (PMMA)-QC-20, and cold-cured-PMMA-Meliodent. The doses and durations: 25, 50 and 100µg/ml, 5, 10, 20, 30 minutes. For Cell viability (CV) MTT was used. 100µg/ml was most effective dose for C. albicans, S.gordonii, and A. actinomycetemcomitans were in heat-cured-PMMA and polyamide for S.mutans. For polyamide, lowest CV was 43% in S.mutans and A.actinomycetemcomitans. CV of heat-cure and cold-cure PMMA were 31% and 32% in S.gordonii, respectively. CV was similar for all resins and durations in S.mutans and A.actinomycetemcomitans and for polyamide for C.albicans and for heat-cure PMMA for S.gordonii. 30-min ozone application killed 80% of all microorganisms in all resins except for C.albicans in polyamide (65% cell death) and cold-cure PMMA (57% cell death). Optimal dose/duration combination was 100 µg/ml-10 min. Gaseous ozone can be considered as an effective cleansing agent for denture base resins.