CRISPR/Cas9 System, an Efficient Approach to Genome Editing of Plants for Crop Improvement

Delpasand Khabbazı S., Delpasand Khabbazi A., Cevik V., Ergül A.

in: RNA-Based Technologies for Functional Genomics in Plants , Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh, Editor, Springer Nature, Chur, pp.369-391, 2021

  • Publication Type: Book Chapter / Chapter Research Book
  • Publication Date: 2021
  • Publisher: Springer Nature
  • City: Chur
  • Page Numbers: pp.369-391
  • Editors: Guiliang Tang,Sachin Teotia,Xiaoqing Tang,Deepali Singh, Editor
  • Yozgat Bozok University Affiliated: No


Crop production in agriculture is affected by plant genetic background and environmental factors. The application of modern molecular biology tools to conventional plant breeding approaches has facilitated the plant genetic improvement attempts. After the extensive employment of recombinant DNA technology in diverse plant species and despite achievements, the use of transgenic crops has been encountered with public concerns due to the presence of transgenes. The advent of sequence-specific nuclease-based editing technologies especially clustered regularly interspaced short palindromic repeat-associated protein system (CRISPR/Cas) has opened a promising avenue in genetic engineering of plants. The importance of this approach is emphasized since it is a simple and robust tool, moreover, non-transgenic mutants can be selected in later generations. Following the successful use of the CRISPR/Cas9 editing tool in model plants, the applications of this system have been increasingly reported in different plant species. This chapter reviews the contribution of the CRISPR/Cas9 system in the development of genetically modified crops with improved yield, nutritional value, and response to biotic and abiotic stress factors.