Development and linkage mapping of novel sex-linked markers for marker-assisted cultivar breeding in pistachio (Pistacia vera L.)


Khodaeiaminjan M., YAŞA KAFKAS N. E., GÜNEY M., KAFKAS S.

MOLECULAR BREEDING, vol.37, no.8, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 8
  • Publication Date: 2017
  • Doi Number: 10.1007/s11032-017-0705-x
  • Journal Name: MOLECULAR BREEDING
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Pistachio, Sex locus, Linkage mapping, Single nucleotide polymorphism (SNP), Simple sequence repeats (SSR), Sex chromosome, PRIMITIVE Y-CHROMOSOME, MALE-SPECIFIC REGION, GENETIC DIVERSITY, GENUS PISTACIA, SSR MARKERS, MICROSATELLITE MARKERS, RAPD MARKERS, IDENTIFICATION, ANACARDIACEAE, PAPAYA
  • Yozgat Bozok University Affiliated: Yes

Abstract

The dioecious character of Pistacia vera L (the pistachio tree) limits its breeding capacity. Thus, early stage selection of males can save time, labor, and land. This study aimed to develop sex-linked single nucleotide polymorphism (SNP) markers, together with expressed sequence tag-derived simple sequence repeats (EST-SSRs), to determine position of the sex locus in pistachio by constructing a linkage map of its sex chromosome for the first time. Nine novel sex-linked SNP markers were successfully identified by SNaPshot minisequencing analysis of 25 SNP loci from 17 restriction site-associated DNA (RAD) reads in 309 individuals. All nine markers were heterozygous in females and homozygous in males supporting a ZW/ZZ sex determination system in pistachio. A total of 105 segregating SSRs and sex-linked markers were used to identify the sex chromosome and the position of the sex locus through analysis of a Siirt x Bagyolu F-1 population with 122 progenies. Of these 105 markers, four common and four paternal SSRs were mapped onto the sex chromosome, along with the phenotypic sex locus and sex-linked markers. The resulting consensus map had a total length of 65.19 cM. The sex locus and sex-linked SNP markers were located in the center of the chromosome at a distance of 31.86 and 31.92 cM, respectively. This study presents valuable information about the sex chromosome and sex locus position as well as novel polymorphic EST-SSRs and nine sex-linked SNP markers in pistachio.