Research Information System
8th Asia-Pacific Chromosome Colloquium, Tekirdağ, Turkey, 18 September 2023 - 21 July 2024, pp.43, (Summary Text)
The Onobrychis genus belongs to the Hedysareae tribe within the Fabaceae
family. The genus includes species with basic chromosome numbers of seven or
eight, existing as diploid (2n = 2x = 14, 2n = 2x = 16), tetraploid (2n = 4x =
28, 2n = 4x = 32) or octoploid (2n = 8x = 56) species ¹. The sainfoin needs to be improved
in terms of genetic studies and has great potential for agriculture. Therefore,
comparative genetic information about the Onobrychis genus is valuable for germplasm
use in breeding. CenH3 is a variant of histone 3 protein and is involved in the
assembly of the kinetochore. It is essential for phylogenetic analysis and
elucidating the evolutionary process of the centromere ³. An earlier study recognized five different CenH3 genomic loci
in O. viciifolia ² .Parallel to this, the genomic sequences of the CenH3 genes in a set of
wild species, O. altissima, O. biebersteinii, O. hajastana, and O.oxydanta are determined by molecular
cloning in our study. Using gene-specific primers, PCR fragmentation was
detected between 4 and 7 in wild species. A detailed analysis of the results
will be included in our poster. Our research, presented here, will contribute
to the comparative analysis of both cultivated and wild-related species with
unique outputs. Additionally, we will discuss the phylogeny and evolutionary
conservation of the CenH3 gene in the Onobrychis genus. This study is supported by
Graduate Thesis Projects (LÜTEP) TGT 2022/22-LÜTEP Niğde Ömer Halisdemir University.