Raspberry, which belongs to the genus Rubus of the Rosacea family, is an important berry fruit with economical potential due to its benefits in terms of human health and wide usage area in industry. In this study, the effects of biological fertilizers (PGPR and mycorrhiza) on some chemical properties of Heritage raspberries were investigated. For this purpose, chlorophyll, anthocyanin, total phenolic content, proline, total carbohydrate levels of plant leaves were evaluated. The highest chlorophyll and anthocyanin contents were obtained from BF5 and BF4 bacterial inoculations (37.24 and 37.85 and 5.58 and 5.14, respectively). The effect of applications on total carbohydrate content was not significant. The highest phenolic and proline contents (2.94 GAE mg g(-1) and 0.091 proline g(-1)) were obtained from BF5 treatment. The results indicated that bacterial applications were the first application in terms of the parameters examined for Heritage raspberry cultivation in the region.