Akademik Veri Yönetim Sistemi
3. uluslararası kanser günleri, Sivas, Türkiye, 15 Eylül - 17 Ekim 2022, ss.128-129
Introduction: The main methods used for cancer treatment can be listed as surgery,
chemotherapy, radiotherapy, and hormone therapy. However, due to the fact that these methods
have some side effects, and the treatments take a long time, patients may go into other searches.
An important part of these studies is the strengthening of the antioxidant defense system against
cancer-related oxidative stress. Previous studies have reported that melatonin has antitumoral
effects against various cancer cells. Although the effect of melatonin has been proven for
various cancer cells in the literature review, we see that there are not enough studies on its effect
on Ehrlich Ascites Tumor. And the fact that the mechanism of action in the cellular dimension
has not been studied in sufficient detail in the studies is one of the issues that need to be clarified.
Methods: EAT cells originating from mouse adenocarcinoma arose spontaneously in a
female mouse in 1905. It was subsequently transplanted from a mouse to a mouse
simultaneously by Ehrlich and Apolant to become an experimental tumor model. In this study,
the effect of melatonin on Ehrlich acid tumor (EAT) cells; Different incubation times (24 and
48 hours) were tested in the 25, 50, 75 and 100 µg/ml dose range. Viable - dead cell, early - late
apoptotic cell and total apoptotic cell concentration (cells/ml) were determined as a percentage
by Annexin V and Dead Cell Test.
Results: When the number of viable cells was evaluated, there was a significant increase
(p<0.005) in the M100 group compared to the control group after 24 hours of incubation. After
48 hours of incubation, viable cell count was lower in M25 (p<0.05), M75 (p<0.001), M100
(p<0.005) groups compared to control group. When the data at the end of the 24-hour incubation
were evaluated, it was observed that the number of early apoptotic cells in the M100 group was
significantly lower than the control group (p<0.005). At the end of 48 hours, there was a
significant increase in the number of late apoptotic cells in the M25 and M75 (p<0.005) groups
compared to the control group. When the 24-hour data were evaluated, a significant decrease
was observed in the total number of apoptotic cells in the M100 group compared to the control
group (p<0.05). After 48 hours, there was a significant increase in the total number of apoptotic
cells in the M25 (p<0.05), M75 (p<0.005) and M100 (p<0.05) groups compared to the control
Conclusion: Our study showed that Melatonin has an anticarcinogenic effect in cancer.
We hope that with the evaluation made in the cell culture study, we have provided useful
information to the literature and our study will be a reference for future studies.