Investigation of the Effects of Medicago sativa L. Methanol Extract on Mortality, Acetylcholinesterase Activity and Oxidative Stress Parameters in Galleria mellonella Larvae
11. INTERNATIONAL CONGRESS ON CONTEMPORARY SCIENTIFIC RESEARCH, Finlandiya, 01 Temmuz 2026, Helsinki, Finlandiya, 1 - 07 Temmuz 2026, ss.516-522, (Tam Metin Bildiri)
- Yayın Türü: Bildiri / Tam Metin Bildiri
- Basıldığı Şehir: Helsinki
- Basıldığı Ülke: Finlandiya
- Sayfa Sayıları: ss.516-522
- Yozgat Bozok Üniversitesi Adresli: Evet
Özet
Biopesticides derived from plants
are environmentally safe substitutes for chemical pesticides. This study
investigated how the methanol extract of Medicago sativa L. (alfalfa)
affects the larvae of the wax moth (Galleria mellonella) by measuring
mortality rate, acetylcholinesterase activity and oxidative stress factors.
Larvae were treated with varying concentrations of methanol extract and after
24, 48 and 72 hours of exposure, larvae were collected for biochemical
analysis. Larvae that had been treated with methanol extracts had significantly
higher mortality rates than control larvae, in a concentration-dependent
manner. Treating larvae with methanol extracts produced statistically
significant changes in acetylcholinesterase activity following exposure,
suggesting that the extracts may have neurotoxic properties. Analysis of
oxidative stress indicated that larvae treated with methanol extracts exhibited
greater amounts of lipid peroxidation and alterations in the parameters of
their antioxidant defense system. Our results indicate that methanol extracts
of Medicago sativa are likely to exhibit significant biopesticidal
activity against Galleria mellonella via the induction of oxidative
stress and through mechanisms mediated by neurophysiology.