Submerging faba beans in water improve in vitro rooting of microprpagated shoots

Çetin Özkan G., KHAVAR K. M.

Journal of Applied Biological Sciences, vol.11, no.1, pp.1-4, 2017 (Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 11 Issue: 1
  • Publication Date: 2017
  • Journal Name: Journal of Applied Biological Sciences
  • Journal Indexes: Scopus
  • Page Numbers: pp.1-4
  • Keywords: In vitro, Mass propagation, Rooting, Submerging, Vicia faba
  • Yozgat Bozok University Affiliated: Yes

Abstract

A reliable shoot regeneration system for faba bean (Vicia faba L.) has not been reported to date to build a strong base for genetic transformation in broad bean. The present work reports a rapid, reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on MS medium containing 0.25 mg/l BAP +0.50, 0.25, 0.10, 0.05, 0.00 mg / l NAA following submerging of the seeds in distilled water for 24 hours. The explants showed a fast regeneration in 15 days time. These shoots were rooted on MS medium containing 1 mg/l IAA (15 d) and acclimatised on soil peat moss (10 days). The plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. This protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions.